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The Southampton six colours

Six synthetic food colours were tested together in a 2007 study at the University of Southampton, and foods containing them now carry a warning in the UK and EU. Here is a reference card for each of the six: what colour it gives, its E number, and how its treatment differs around the world.

Diet, allergens and labels 2 minute read Updated 26 September 2026 7 sources

In this article
  1. The six, one by one
  2. The warning
  3. What the regulators concluded
  4. Choosing sweets without them

The Southampton six are six synthetic food colours that were tested together in a study commissioned by the UK Food Standards Agency and carried out by researchers at the University of Southampton. The study, published in 2007, tested a mixture of the colours along with the preservative sodium benzoate: see why do some sweets have a warning about children?

The six, one by one

| E number | Name | Colour | In the United States | |---|---|---|---| | E102 | Tartrazine | Lemon yellow | Used, as FD&C Yellow 5, with batch certification | | E104 | Quinoline yellow | Greenish yellow | Not a permitted food additive | | E110 | Sunset yellow | Orange | Permitted, as FD&C Yellow 6 | | E122 | Carmoisine, or azorubine | Red | Not listed; delisted in 1963 | | E124 | Ponceau 4R | Strawberry red | Not approved for human consumption | | E129 | Allura red | Red | Permitted, as FD&C Red 40 |

A few notes on individual colours:

  • Tartrazine can be mixed with blue colours to make greens. According to the US Food and Drug Administration, it causes hives in fewer than 1 in 10,000 people.
  • Quinoline yellow is permitted in the EU and Australia but not as a food additive in Canada or the United States, where it is allowed in medicines and cosmetics.
  • Carmoisine was listed in the United States in 1939 for drugs and cosmetics applied to the skin, and delisted in 1963.
  • Allura red was introduced in the United States as a replacement for another red, amaranth.

The warning

Under Article 24 and Annex V of Regulation 1333/2008, food containing any of the six must show the colour's name or E number along with the warning that it may have an adverse effect on activity and attention in children. The same rule applies in the UK.

What the regulators concluded

The European Food Safety Authority reviewed the evidence and in 2009 concluded that the available science did not substantiate a link between the colours and behavioural effects. On the basis of other evidence, it lowered the acceptable daily intake for some of them, such as quinoline yellow, which fell from 10 to 0.5 mg per kg. The UK Food Standards Agency called for voluntary removal of the colours, and the US Food and Drug Administration made no changes after the study.

Choosing sweets without them

Sweets can be coloured with plant and algae colours instead, such as spirulina, black carrot, curcumin and paprika: see natural colours in sweets To check a sweet, look for the six E numbers or names in the ingredients, or the warning itself: see how to read a sweet label

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Questions and answers

What are the Southampton six colours?

Tartrazine, E102; quinoline yellow, E104; sunset yellow, E110; carmoisine, also called azorubine, E122; ponceau 4R, E124; and allura red, E129. They are synthetic colours tested together in a study commissioned by the UK Food Standards Agency.

Are the Southampton six banned?

No. They are still permitted, but in the UK and EU foods containing them must carry the warning that they may have an adverse effect on activity and attention in children. Several are not permitted in food in the United States, while others are.

Did the study prove they cause hyperactivity?

The European Food Safety Authority reviewed the evidence and in 2009 concluded it did not substantiate a link between the colours and behavioural effects, although it lowered the acceptable daily intakes of some of them on other evidence. The labelling requirement remained.

Sources

  1. Tartrazine, Wikipedia
  2. Quinoline Yellow WS, Wikipedia
  3. Sunset yellow FCF, Wikipedia
  4. Azorubine, Wikipedia
  5. Ponceau 4R, Wikipedia
  6. Allura Red AC, Wikipedia
  7. EFSA evaluates Southampton study on food additives and child behaviour, European Food Safety Authority

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